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The Siliciano Laboratory, Division of Infectious Diseases in the Department of Medicine at Johns Hopkins University is seeking a Research Technologist. The Siliciano Laboratory studies the mechanism of persistence in HIV-1 infection. Despite the remarkable efficacy of combination antiretroviral therapy (ART), HIV-1 infection remains incurable with ART alone. This is due to viral persistence in stable reservoirs, the best understood of which is a latent reservoir in resting CD4+ T cells. The latent reservoir in resting CD4+ T cells is the major barrier to cure and is the focus of our laboratory's research efforts. We have recently demonstrated that most of the cells in the reservoir are generated by the proliferation of previously infected cells rather than de novo infection of CD4+ T cells. In addition, we have shown that the latent reservoir is dominated by large clones of infected cells which wax and wane over time. We have also developed a more accurate method of measuring the HIV-1 reservoir that separately quantitates intact and defective proviruses.
Specific Duties & Responsibilities:
The individual will work closely with the Principal Investigators, together with lab technicians and graduate students, on research projects employing the following techniques:
Processing of blood samples from HIV-infected donors in a biosafety level 2+ laboratory
Sterile tissue culture technique in a biosafety level 2+ laboratory
Molecular biology techniques, including qPCR, digital PCR, and sequencing
Maintain well-organized experimental records
Participate in research group meetings
May assist in recruiting blood donors and maintaining relevant supplies, reagents, and cell lines
Will primarily assist with the development and evaluation of new methods for detecting and quantifying latent HIV in infected donors.
Provides an excellent opportunity for a highly motivated individual to develop advanced skills in molecular virology and translational research.
It is ideal for individuals interested in pursuing graduate or medical school after 1-3 years in the laboratory.
Opportunities to present results at scientific meetings or in research publications may be available.
The applicant must be willing to work with blood samples from HIV-infected donors.
Extensive training on safe handling practices will be provided.
Bachelor's Degree in Biology, Chemistry, or a related field is required.
Related post-undergraduate experience or education is a plus.
Prior laboratory experience is highly desirable.
* Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
Special Knowledge, Skills & Abilities:
Competitive candidates will have prior laboratory experience with an emphasis in basic molecular biology and cell biology techniques including, but not limited to: DNA sequencing and analysis, qPCR, cloning, cell culture and primary cell work.
Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $15.00 - $20.00/hr (commensurate with experience) Employee group: Full Time Schedule: Mon-Friday 8:30am-5pm Exempt Status: Non-Exempt Location: School of Medicine Campus - 100% on-site Department name: SOM DOM Infectious Disease Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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