Multi-disciplinary lab focused on regenerative medicine seeks highly motivated ResearchSpecialist/Lab Manager to lead, conduct, and manage a highly interactive group conducting research in human pluripotent and hemato-vascular stem cell biology. The manager will conduct laboratory research by contributing to both ongoing projects with other lab members, as well as in their own individual projects related to human stem cell biology. This position requiresexperience in both lab administration andbasictechniques in molecular biology and tissue culture. The lab is highly collaborative, and located in state-of-the art facilities at the Johns Hopkins School of Medicine Institute of Cell Engineering. Our scientists focus on modifying and reprogramming human cells to answer fundamental questions in tissue engineering. The Institute provides an innovative environment to stimulate collaboration, and accelerate scientific progress: https://www.hopkinsmedicine.org/institute_cell_engineering/
Specific Duties & Responsibilities
Cell culture, maintenance, and gene transfer/editing of human induced pluripotent stem cells (hiPSC) and human embryonic stem cells (hESC)
Reprogramming of normal and diseased human hematopoietic cells and skin fibroblasts to hiPSC for disease modeling
Directed differentiation of hESC, normal and diseased human iPSC to vascular, neural, retinal, and hematopoietic lineages.
Maintain laboratory conditions, reagent ordering, supply inventory, execute proper disposal of biohazardous materials, and other administrative duties of lab manager
Manage animal health and breeding of normal and mutant strains of mice
Develop experimental designs and have primary responsibility for planning, implementation, evaluation and interpretation results; design process may be completed either in collaboration with the PI, or independently.
Coordinates and/or conducts multiple, concurrent assignments or a multi-faceted project, independently or by collaborating with others.
Compile, analyze, and interpret research data. Monitor documentation of results; review and recognize documentation, which may lead to modification and adaptation of research methodologies; adapt and/or modify experimental methods based upon interpretation of data.
Conduct library research; participate in editing and preparing manuscripts, scientific illustrations, and computer graphics for publication.
Oversee and assure laboratory compliance with regulatory requirements and JHU standards, policies and procedures.
Work with other research staff to prioritize and schedule projects.
BS/BA in biological sciences, chemistry or related field
Minimum of 2 years' laboratory experience performing relevant techniques in a comparable setting
Advanced degree may substitute for experience to the extent permitted by the JHU equivalency formula.
* JHU Equivalency Formula: Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
Master's degree in Biotechnology, preferred
Special Knowledge, Skills, and Abilities
Experience with aseptic tissue culture techniques
Must have ability to follow molecular biology protocols accurately and carefully
Ability to maintain detailed experimental records is essential.
Operation of centrifuge, pH meter, analytical balance, incubator, spectrophotometer, light microscope, imaging systems and laminar flow hoods;
Familiarity with theory behind experiments; ability to apply analytical skills to interpret experiments using positive and negative controls.
Excellent interpersonal and communication skills and the ability to work effectively with a wide range of diverse individuals is essential.
Excellent problem-solving, organizational, and analytical skills are required.
Histological and immunofluorescence methods for confocal microscopy of animal and human tissues. Image capture and data analysis of confocal microscopic images using image software
Performance of routine molecular biology techniques including RNA, DNA, and protein extraction, PCR, RT-qPCR, transient transfections for loss and gain of function studies, ELISA, immunoprecipitation, and immunoblot analysis
Experience in training and guiding rotating undergraduate research students is desirable
Special skillsin animal surgery and micro-dissection are highly desired.
Classified Title: Research Specialist II Working Title: Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: $38,920 - $53,520; Commensurate with experience Employee group: Full Time Schedule: Monday-Friday, 8:30am- 5:00pm; Up to 37.5 hrs/week Exempt Status: Exempt Location: 04-MD:School of Medicine Campus Department name: 10002936-SOM Onc Pediatric Oncology Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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