In this exciting role, the Research Assistant will work in the Department of BioEngineering and BioMedical Sciences to join a research core facility for a National Cancer Institute-funded Program Project. It is a multi-investigator, multi-institutional and interdisciplinary effort to define biology-driven protein structures and obtain mechanistic understanding of DNA repair machines for cancer research. The Research Assistant will take part in research and provide services to the program project by supporting production and analysis of high-quality key reagents (including vectors, proteins, and cell products) using advanced gene engineering and recombinant protein expression technology. The Research Assistant will perform standard laboratory protocols and routine experimental procedures of molecular cloning, cell culture and protein biochemistry, and support operation of the core facility with standard lab responsibilities.
This is a Part-time, 24 hour per week, appointment up to 900 hours.
What You Will Do:
Perform work assignments in support of research projects in the core facility.
Perform standard molecular biological, cell biological and biochemistry procedures, that is, plasmid purification, DNA and protein gel electrophoresis, Western blots, recombinant protein expression and analysis, affinity chromatography, bacterial culture, and insect cell culture.
Collect data, understand results, and recognize deviations from established protocols.
Communicate experimental results timely to the Head Scientist for troubleshooting and follow-up experimental design and planning.
Maintain detailed records of experiments and outcomes.
Use computer software to input, process and manage data for further analysis.
Update and maintain standard operating procedures.
Provide general assistance to the team members.
Report project progress in weekly lab meetings and attend annual program workshops.
Maintain a clean and safe working environment and comply with local, state, and federal environmental, health and safety regulation.
Routinely perform basic lab support duties, including preparation of buffers and media, glassware and plasticware cleaning and sterilization, cleaning and maintenance of lab equipment, cleaning and organization of repository freezers, stocking and tracking of consumables, and requisition of waste disposal, etc.
Maintain and update database for reagent depository/distribution center of the core facility.
Pack shipments with reagent labels and inventories to distribute the program members and/or collaborators.
What is Required:
Demonstrated laboratory techniques in molecular biology (such as nucleic acid purification, PCR, DNA cloning, and sequencing), cell biology (such as bacterial culture and insect cell culture), and protein biochemistry (such as protein extraction, SDS-PAGE and WB).
Experience working in a research lab.
Proficient computer skills for documentation and basic analysis (such as Word, Excel, PowerPoint, and sequence analysis, etc.).
Detail-oriented, strong record-keeping ability, excellent organizational and time management skills.
Ability to follow verbal instructions and written lab protocols.
Rudimentary knowledge and skills in biology, chemistry, molecular biology, and biochemistry required to perform tasks associated with specific research assignments.
Demonstrated ability to work independently and in a team environment.
Effective communication skills in writing and verbal presentations.
General knowledge of science laboratory. Strong science foundation and ability to apply concepts for troubleshooting experiments and instrumentation.
Good analytical skills to recognize deviations from established protocols.
Additional Desired Qualifications:
Bachelor's degree in molecular biology, biochemistry, or related fields preferred or equivalent work experience.
Knowledge of environmental, health and safety, security, procurement and business management policies and procedures.
Experience in managing and updating electronic laboratory supply and reagent inventories.
Ability to perform multiple procedures/assays and prioritize work assignments, and coordinate with team members.
The posting shall remain open until the position is filled, however for full consideration, please apply by close of business on November 3, 2020.
This is a temporary, part time (24 hours per week), limited appointment with the possibility of renewal based upon satisfactory job performance, continuing availability of funds and ongoing operational needs. Cannot exceed 900 hours within a rolling 12-month period.
Non-exempt (hourly paid) from overtime pay.
This position is represented by a union for collective bargaining purposes.
Salary will be determined based on range by collective bargaining agreement.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed at: West Berkeley Biocenter (Potter St.) -- Bldg. 977, 717 Potter St., Berkeley, CA.
Learn about us!
Biological Systems and Engineering Division
Vision: To lead efforts that combine the power of biology with the tools of engineering to develop sustainable energy and biomanufacturing solutions and improve human health.
Mission:Advance a mechanistic and predictive understanding of complex biological systems over multiple scales in terms of their responses to manipulation, stress, disease and environmental challenges and translate this knowledge using engineering principles to develop resilient systems, tools, and processes for the efficient production of fuels, chemicals, materials, tissues, and therapeutics.
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Working at Berkeley Lab has many rewards including a competitive compensation program, excellent health and welfare programs, a retirement program that is second to none, and outstanding development opportunities. To view information about the many rewards that are offered at Berkeley Lab- Click Here.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law.
Lawrence Berkeley National Laboratory encourages applications from women, minorities, veterans, and other underrepresented groups presently considering scientific research careers.
Internal Number: 91329
About Lawrence Berkeley National Laboratory
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.