Technical support is needed for a small research group with a longstanding focus on the development of new treatments for tropical diseases. This includes the identification of new drug targets as well as the testing of newly synthesized compounds and drug combinations for activity against malaria parasites and trypanosomes. Under the general supervision of a faculty investigator the Research Specialist is responsible for performing established assays and participating in general maintenance of the laboratory, equipment and supplies. Standard techniques of molecular pharmacology, molecular biology, and biochemistry are utilized, as are more innovative approaches with, for example, RNAi, in vitro pharmacokinetics/pharmacodynamics (PK/PD), and quantitative LC-MS/MS.
Specific Duties & Responsibilities
Maintain Plasmodium falciparum, Trypanosoma brucei, and some mammalian cells in tissue culture; prepare stabilates
Test new compounds in vitro against cells
Maintain, sterilize, assemble and run in vitro cartridge system for determining pharmacokinetic/pharmacodynamic relationships
Compile, process and record data; cross validate and prepare data for publication
Order lab supplies through online SAP system, and reconcile orders with receipts
General lab maintenance, including inventories, prepare stock solutions, autoclave supplies, fulfill radiation safety requirements, service maintenance for equipment and distilled water, ensure orderly disposal of biohazardous waste
Maintain lab methods books and inventory of frozen cell lines
Opportunity to learn and conduct quantitative UPLC/MS-MS assays of drugs in tissue culture medium and mammalian tissues
Essential Job Functions
Meticulous technique in cell culture, sometimes repetitive and tedious, required. Must adhere to established methods unless modifications are specifically approved. Must be willing and able to handle radioisotopes. Must like and be good at basic math: able reliably to calculate molar solutions, and to utilize pre-designed spreadsheets to obtain pharmacokinetic parameters. Must be willing to attend and present at lab meetings.
Other Job functions
Place lab supply orders through the Shopping Cart function in SAP/Hopkins 1. Reconcile invoices/receipts for lab supplies.
Bachelor’s degree in biological sciences or related field. Additional education may substitute for some experience, to the extent permitted by the JHU equivalency formula.
* JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for the required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job. *
Requires a minimum of 1 year of previous experience in a lab required. Experience with cell culture, computer spread sheets, graphics, word processing required. Must be accurate and neat in work. Must be reliable and willing to learn new skills. Must be able to operate photocopier, scanner, and fax machine.
Experience with pathogens preferred. Experience in handling animals and radioisotopes. Prefer but do not require, experience with basic methods of molecular biology and biochemistry, as well as with genome databases and genomic/proteomic software.
Special Knowledge, Skills, and Abilities
Strong verbal and written communication skills necessary as are excellent organizational skills. Familiarity with basic computer programs, i.e., Word, Excel.
Must have the interest and ability to tackle new, sometimes challenging, projects. Ability to maintain an orderly lab environment; cooperative nature that supports multiple lab users and new trainees.
Reports to: Elizabeth Nenortas, Senior Research Specialist
Licensure, certificate, registration: Online Hopkins training in biohazards, radiation safety
Sitting in a normal seated position or standing for extended periods of time. Working at a computer terminal for extended periods of time. Reaching by extending hand(s) or arm(s) in any direction. Lifting and transferring incubator tray with pharmacokinetic/pharmacodynamic apparatus between incubator and sterile cabinet. Finger dexterity to manipulate objects with fingers rather than with whole hand or arm, for example, using a keyboard or pipetting solutions. Communication skills using the spoken word. Good reading, writing, and typing skills. Able to carry notebooks, supplies needed for experiments. Able to transport sterile supplies, notebooks on a wheeled cart between laboratories, equivalent to walking 1-2 blocks, sometimes several times per day. Ability to see within normal parameters. Ability to hear within normal ranges.
Classified Title: Research Specialist Working Title: Research Specialist Role/Level/Range: ACRP/03/MA Starting Salary Range: $33,545 - $46,095; Commensurate with experience Employee group: Full Time Schedule: Monday - Friday, 8:30am-5:00pm / 40 hours per week Exempt Status: Non-Exempt Location: 04-MD:School of Medicine Campus Department name: 60005229-SOM DOM ClinPharm Shapiro Lab Personnel area: School of Medicine
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