The Research Specialist II will work in the Division of Gastroenterology and will interface with members of this and other labs in the Division. The position will utilize a solid foundation in basic laboratory principles and techniques associated with molecular and cell biology to perform the required tasks. A qualified applicant should be capable of exercising independent judgment while participating in the planning of experiments and evaluating results. When needed, he or she will also provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis.
The Research Specialist II will work in a laboratory that focuses on the pathogenesis of inflammatory bowel disease (IBD). The Research Specialist II will utilize a solid foundation in basic laboratory techniques to maintain all animal protocols, mouse colonies, laboratory equipment, reagent inventories, and assist in routine experimental work like plasmid preparations, cell culture and purification of recombinant proteins. The work will also involve maintaining enteroid cultures, develop and use enteroid platforms, use bacterial to develop models of host-pathogen interactions. The Research Specialist II has experience with cell handling and is capable of exercising independent judgment while participating in the planning of experiments and evaluating and interpreting results.
Specific duties & responsibilities:
Participates in planning studies and evaluating/interpreting test results utilizing detailed knowledge of research laboratory procedures and practices involving intestinal epithelial cells. These lab-based duties will include:
Isolation and manipulation of human and mouse enteroids
Independent troubleshooting of technical and experimental issues
Selects, develops, performs and evaluates research methods; operates appropriate equipment.
Uses sterile techniques to avoid contaminating lab experiments. Be knowledgeable of and employ good tissue culture techniques.
Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports.
Is an integrated member of the research team. May be involved in establishing laboratory protocols. Will be asked to prepare reports on the analysis.
Exercises professional and scientific judgment and assumes responsibility for decisions, consequences, and results. Engages in regular and continuous decision making having an impact on the success of the laboratory.
Exchanges routine information in an appropriate manner requiring good oral and written communication skills. Has operating knowledge of the personal computer and has the ability to search and retrieve information on the role of extracellular matrix proteins in inflammation and fibrosis from the internet.
Minimum qualifications (mandatory):
Bachelor’s degree in biology, chemistry, or related field plus two years of laboratory experience performing relevant techniques. Master’s degree, with related graduate research may substitute for experience.
Related Master's degree or higher
Special knowledge, skills, and abilities:
As noted above.
Technical qualifications or specialized certifications:
As noted above.
Any specific physical requirements for the job:
Sitting in a normal seated position for extended periods of time. Ability to use a keyboard and move about. Ability to see and hear within normal parameters.
Classified Title: Research Specialist II Working Title: Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: $38,920 - $53,520 Employee group: Full Time Schedule: M-F. 8:30-5:00 Exempt Status: Exempt Location: 04-MD:School of Medicine Campus Department name: 10002808-SOM DOM Gastroenterology Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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