Will be responsible for sample handling, planning and executing molecular genetics tests (i.e., genotyping or sequencing) on large numbers of DNA samples.
Barcode and enter sample associated information into the database.
Transfer DNA samples into 96-well plate format.
Receive and prepare deepwell DNA stock plates and create working DNA plates.
Determine concentration and volume of DNA samples using various instruments.
Dilute samples to working concentrations.
Maintain and monitor inventory of all in-house DNA samples and reagent supplies.
Create master mixes; Set up polymerase chain reactions; trouble-shoot such reactions.
Operate and troubleshoot robotic liquid handlers.
Prepare and complete SNP genotyping assays including, but not limited to, Illumina assays and workflows.
Prepare samples for targeted capture of specific regions of DNA for sequencing experiments. This includes library preparation and capture techniques.
Prepare and complete Next-Gen sequencing assays including, but not limited to, Illumina HiSeq workflows.
Perform quality control checks of data using various software, including, but not limited to, GenomeStudio, Spotfire and other in-house QC metrics reports.
Maintain timely and accurate laboratory records (electronic and written).
Collect, organize, interpret and submit experimental data to database and supervisors.
Perform general lab maintenance duties.
Perform validation tests of lab reagents.
Participate in committees that function to improve processes.
Communicate effectively to supervisor and team members.
Perform miscellaneous related duties as assigned.
Bachelor’s degree in Biology, Chemistry, or equivalent in biological sciences is required.
Six months of post-undergraduate experience in a comparable lab or significant research project, not related to normal course requirements.
Special Knowledge, Skills, and Abilities:
Basic understanding of molecular biology and genetics.
Requires the ability to safely operate scientific laboratory equipment.
Requires flexible work hours beginning as early as 6:00 a.m. and/or ending as late as 6:00 p.m.
The job requires superior organizational skills for time and data management.
Ability to function well in a team production setting is necessary.
Centrifuges, Illumina BeadLab system (Tecans, iScans, HiScans, TeFlow), Illumina BeadXpress, Covaris, BioAnalyzer, Nanodrop instruments, Illumina HiSeq, SpriWorks, thermal cyclers, robotic and manual liquid handlers, common laboratory equipment, Macintosh, PC computers, UNIX servers, Oracle database.
Classified title: Research Technologist
Working title: Research Technologist
Role/Level/Range: ACRO 40/E/02/CC
Starting Salary Range: $14.14 - $19.44 (commensurate with experience)
Employee group: Full time
Employee Status: Non-Exempt
Schedule: Monday-Friday - 40hrs/Wk - Work shift may vary between the hours of 6AM- 6PM.
Location: Suite 200 - 1812 Ashland Ave, Baltimore, MD – East Baltimore
Department name: 10002884-SOM Genetics Ctr for Inhrtd Dis Res CIDR - IGM
Personnel area (School): SOM – School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at email@example.com. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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