Under general supervision, performs research on HIV, HCV, COVID-19, and HSV-2 in a Bio Safety Level 2 setting. Main research focus are the HIV+ to HIV+ Transplantation and the COVID-19+ Convalescent Plasma studies. The candidate will need to follow established procedures and protocols utilizing appropriate techniques to perform laboratory tests and/or experiments which may include: whole blood and tissue processing, ELISAs, DNA and RNA extractions, amplification by RT-PCR, and data analysis. The individual will participate in the planning of experiments and evaluating and interpreting results. Exchange of routine information in an appropriate manner requiring good oral and written communication will be necessary. Regular interaction with students, vendors, post-doctoral fellows, faculty members, and other clinical fellows will occur. There will also be responsibility for routine operational activities such as; reviewing inventory, maintaining up to date records of tests, preventative maintenance of equipment, etc.
Specific Duties and Responsibilities:
Follows established protocols and utilizes aseptic technique to perform laboratory experiments which include: whole blood processing, tissue processing for microscopy, antibody testing by ELISA and Western blot, molecular biology including RNA and DNA extraction from HIV infected blood and tissue, PCR techniques including RT-nested PCR, sequence analysis, viral loads.
Maintains up-to-date computer records for samples in the freezer inventory and databases. Keeps lab notebook with records for all experiments.
Operates and maintains basic laboratory equipment such as thermocyclers, pipettes, centrifuges, freezers, incubators, light microscope, and Bio Safety Cabinets.
General Laboratory upkeep for cleanliness and safety.
Ships supplies to active study sites. Receives, process and documents samples from active sites.
Performs miscellaneous related duties as assigned.
Minimum Qualifications (mandatory):
Bachelor’s degree in biological sciences, chemistry, or related field required.
Knowledge of good lab practices required.
Some related research laboratory experience strongly preferred.
Special Knowledge, Skills, and Abilities:
Good oral and written communication skills.
Must have excellent organizational skills.
Ability to work independently, as well as part of a team.
Knowledge of Microsoft Excel.
Specific physical requirements for the job:
Equipment, Machine, or Tool Requirements:
Computer and Laboratory equipment
Uses various software applications, such as Excel, email, statistical packages (STATA), databases (Freezer Works), and graphics packages (Graph Pad).
Work Environment /While performing the duties of this job:
While performing the duties of this job, the employee may remain in a stationary position sitting or standing. Occasionally required to traverse throughout the laboratory or to retrieve equipment, supplies, or specimens from other locations on campus. The employee is required to operate computer keyboard, laboratory instruments, and small laboratory tools such as pipettes, cutting instruments, glass tubes, dishes, and/or slides. Constantly repositions self to perform duties at laboratory work stations, gathering specimens, and loading equipment.
Prolonged vision requirements including viewing computer screen, paper reports/documents, charts and results. Visual acuity is required to distinguish fine gradation of color or structure and closely examine specimens (including via a microscope).
Laboratory environment - Exposure to toxins and infectious agents exist, but potential for personal injury or harm is minimized if established safety and health precautions are followed. Must refer to and are expected to comply with procedure manuals, follow proper laboratory protocols and safety policy/procedures, and be familiar with material data safety sheets in assigned work areas. Able to read and understand all Health, Safety and Environment (HSE) guidelines applicable to assigned work area. Working in laboratory where there may be discomforts due to odors, noise, temperature fluctuations, and working around lab equipment. Research areas may include animal, human, human products, DNA, radiation protocols, and other bio-hazardous materials. Use of personal protective equipment may be advised or required.
May transport equipment and supplies usually less than 40 pounds from one area to another (in the laboratory or on campus (specify)). May be required to lift and/or move up to 50 pounds with proper training, or precautions/lifting aides (example: supply boxes or Formalin cubes). In some areas work space is limited.
Staff may be required to complete annual competency review, and must insure compliance with Johns Hopkins Policy, Laboratory Policy & Procedure, Health, Safety & Environment regulations, and all applicable privacy & confidentiality laws/practices.
Many department laboratories operate 24/7 with schedules on day, evening, or night shift. Rotating schedules which may include weekend work and holiday assignment. (Overtime possible when authorized/needed to meet business and patient needs).
Classified Title: Research Technologist Working Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $14.14-$19.44 (commensurate with experience) Employee group: Full Time Schedule: M-F 9:00am-5:30pm Exempt Status: Non-Exempt Location: 04-MD:School of Medicine Campus Department name: 10003067-SOM Pat Transfusion Medicine Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at email@example.com. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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