The School of Medicine is seeking a Sr. Research Specialist.Technical support is needed for a small research group with a longstanding focus on the development of new treatments for tropical diseases. This includes the identification of new drug targets as well as the testing of newly synthesized compounds for activity against malaria parasites and trypanosomes. Under the general supervision of a faculty investigator the Research Specialist is responsible for independently developing and performing assays, supervising two research specialists, guiding student trainees, and overseeing general maintenance of the laboratory, equipment and supplies. Standard techniques of molecular pharmacology, molecular biology, and biochemistry are utilized, as are more innovative approaches with, for example, RNAi, in vitro pharmacokinetics/pharmacodynamics (PK/PD), and quantitative LC-MS/MS.
Specific Duties & Responsibilities
Maintain Plasmodium falciparum, Trypanosoma brucei, and some mammalian cells in tissue culture; prepare stabilates
Test new compounds in vitro against malaria parasites and trypanosomes
Maintain, sterilize, assemble and run in vitro cartridge system for determining pharmacokinetic/pharmacodynamic relationships. Develop and test new apparatus for two-drug dosing regimens.
Direct supervisory responsibility for two research technologists and all other staff and trainees, in routine lab maintenance and safety procedures, sterile tissue culture technique
Learn and conduct quantitative UPLC/MS-MS assays of antiparasitic drugs in tissue culture medium, in support of PK/PD studies
Compile, process and record antiparasitic data; cross validate data before publication
Prepare radiolabeled DNA probes; northern and Southern blots
Protein determinations, enzyme assays, SDS-PAGE, protein transfer and western blot analysis
Basic molecular biology skills: DNA, RNA purification; cloning; PCR; expression and purification of recombinant protein; obtaining and validating DNA sequence
Infect rats and mice with trypanosomes; treat monitor, and sacrifice rats and mice
General lab maintenance, including ordering, inventories, prepare stock solutions, autoclave supplies, maintain darkroom facilities, fulfill radiation safety requirements, service maintenance for equipment and distilled water, ensure orderly disposal of biohazardous waste
Negotiate best prices for purchases and service agreements
Interact with JHU Inventory Office to maintain up-to-date equipment inventory
Interact with SPH (Sullivan Lab and others within the Malaria Research Institute) regarding inter-connected work with the Shapiro Lab.
Maintain lab methods books
Maintain records of lab personnel training in radiation safety, animal handling, and biohazards
Prepare work space and orient new lab personnel to facilities and expected procedures
Bachelor’s degree in biology, chemistry or related field required.
Four years of experience in a comparable laboratory performing relevant techniques.
Experience with cell culture strongly preferred; experience with computer spread sheets, graphics, word processing required. Must be accurate and neat in work. Must be reliable and willing to learn new skills. Must be able to operate photocopier, scanner, and fax machine.
Essential Job Functions
Must like and be good at basic math: able reliably to calculate molar solutions, and to utilize pre-designed spreadsheets to obtain pharmacokinetic parameters.
Other Job functions
Place lab supply orders through the Shopping Cart function in SAP/Hopkins 1
Research technologist, all new trainees and personnel in the lab, regardless of position or rank.
Prefer, but do not require, experience with genome databases and genomic/proteomic software; prefer some experience in handling animals and radioisotopes.
Special Knowledge, Skills, and Abilities
Must have the interest and ability to tackle new, sometimes challenging, projects. Ability to organize and maintain an orderly lab environment; cooperative nature that supports multiple lab users and new trainees.
Sitting in a normal seated position or standing for extended periods of time. Working at a computer terminal for extended periods of time. Reaching by extending hand(s) or arm(s) in any direction. Finger dexterity to manipulate objects with fingers rather than with whole hand or arm, for example, using a keyboard or pipetting solutions. Communication skills using the spoken word. Good reading, writing, and typing skills. Able to carry notebooks, supplies needed for experiments. Able to transport sterile supplies, notebooks on a wheeled cart between laboratories, equivalent to walking 1-2 blocks, sometimes several times per day. Ability to see within normal parameters. Ability to hear within normal ranges.
Classified Title: Sr. Research Specialist Working Title: Sr. Research Specialist Role/Level/Range: ACRP/04/MC Starting Salary Range: $45,195 - $62,225; Commensurate with experience Employee group: Full Time Schedule: Monday-Friday, 8:30am-5:00pm, 37.5 hours/week Exempt Status: Exempt Location: 04-MD:School of Medicine Campus Department name: 10002806-SOM DOM Clinical Pharmacology Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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