The Polotsky laboratory is seeking a highly motivated individual to work in the laboratory on a project studying the effect of air pollution (particulate matter) on airway reactivity and lung function and modifying role of concurrent obesity and sleep apnea.
Our laboratory is renowned for studies of the pathogenesis and complications of sleep apnea in the mouse model. This project is performed in collaboration with laboratory of Wayne Mitzner, who is an internationally recognized expert on physiology of asthma and low airways in the mouse model.
Under the general supervision of a faculty investigator or laboratory supervisor, is responsible for performing routine analyses and tests on animal or human fluids and/or tissue or cultured cell lines. Work requires basic use of scientific methods, procedures and techniques gained through previous training and experience. Uses basic understanding of theory behind experiments and applies basic analytical skills to implement assigned projects, and to identify and trouble-shoot situations needing special attention.
Specific duties & responsibilities:
Follows established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments which may include: basic tissue culture, cell separation methods, electrophoretic separations, assays, basic molecular biology, or other specialized histology and biological science techniques. (This statement includes a wide variety of procedures which may be performed under this title in different laboratories throughout the University. Seldom would all be found in any one position.)
1) Operates basic laboratory equipment such as centrifuge, pH meter, analytical balance, incubator, scintillation counter, spectrophotometer, light microscope, and/or utilizes laminar flow hood. Operates lab computers for data entry.
2) Actively participate in conduct of animal experiments to generate controlled dietary and PM model. Maintain intraoperative records and maintains health records in accordance with USDA and AALAC guidelines.
3) Monitor animals in the pre and postoperative period and maintain postoperative records. Perform necropsies to collect tissue and fluid specimens for analyses.
4) Bleeds and injects animals according to protocols
5) Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports.
6) Maintains and makes minor adjustments to equipment, e.g. calibrating, alignment.
7) Complies with biohazard/radiation safety standards through proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace.
8) Completes annual university biohazard/universal precaution/radiation safety training, as appropriate.
9) Uses sterile techniques to avoid contaminating lab experiments.
10) Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.
Minimum qualifications (mandatory):
Required: BS/BA, or equivalent, in biological sciences, chemistry, or physics
Preferred: Six months of post-undergraduate experience in a comparable laboratory, or a significant independent research project, not related to normal course requirements. Comparable laboratory experience may be substituted for educational requirement.
Special knowledge, skills, and abilities:
Scope of Responsibility: Knows the informal policies, procedures and practices necessary to conduct the normal function of a specific section, unit, or work area. Is aware of the role of the position and its potential impact on the working unit.
Decision Making:Carries out duties and responsibilities with limited supervision. Makes decisions and establishes work priorities on essentially procedure-oriented operations.
Communication: Exchanges routine information in an appropriate manner requiring good oral and written communication skills.
Any specific physical requirements for the job:
Work produced is subject to precise measures of quantity and quality Work environment may include areas of unpleasant extremes of cold or heat Biohazardous conditions such as the risk of radiation exposure, fumes or airborne particles, and/or toxic or caustic chemicals may be present in this work environment which mandate attention to safety considerations Near vision to see objects clearly within 20 inches Sharp focus to adjust vision when doing close work that changes in distance from eyes Full spectrum vision to identify and distinguish color Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s), for example, using a keyboard Handling by seizing, holding, grasping, turning or otherwise working with the hand or hands, but without finger dexterity Sitting in a normal seated position for extended periods of time Occasionally lifting, carrying objects weighing 10 lbs. or less Occasionally pushing, pulling objects weighing 30 lbs. or less Ability to move about
Classified Title: Research Technologist Working Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $14.14-$19.44 Employee group: Full Time Schedule: M-F; 8:30am-5:00pm Exempt Status: Non-Exempt Location: 33-MD:Johns Hopkins Bayview Department name: 10002817-SOM DOM Pulmonary Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at email@example.com. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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