1. Function as an integral member of a team investigating mechanisms of pain and hypersensitivity, with studies spanning animal models of migraine, visceral pain, chemotherapeutic-induced peripheral neuropathy, post-operative pain, and persistant inflammatory pain, to the study of tissue of patients suffering from these pain conditions.
2. Participate in a variety of molecular, cellular, and immunohistological methods including cell culture, genotyping and maintaining knockout mice, single cell RT-PCR, ELISA, flow cytometry, protein quantification, western blotting and fluorescence microscopy.
3. Perform a variety of immunohistological and biochemical assays, including identification of suitable strategies for visualization of targets of interest, verification of antibody specificity and establishing ELISAs for protein quantification, and interpret the results.
4. Trouble shoots methods and optimizes protocols.
5. Keep precise records and complies results and assemble them in presentable formats (graph, plots, tables, etc.).
6. Attend and present at local scientific meetings including lab meetings and seminars.
7. Instruct and assist undergraduate, graduate and postdoctoral fellows in methods of immunohistology, ELISAs, western blots PCR and flow cytometry/FACS.
8. Function as a lab manager, including oversight of following functions: a. Order supplies for the laboratory. b. Develop and maintain/monitor budgets for ongoing experiments, contracts and grants. c. Maintain chemical/supply inventory. d. Maintain records and signage, PPE, or other safety equipment and supplies as well as training records of laboratory personnel required by University regulations (IACUC, EHS, DEA, etc). e. Maintain laboratory equipment/monitor service contracts.
9. Other duties as assigned.
Work location: 203 Lothrop Street, Suite E1440, Pittsburgh, PA 15213
If interested, submit resume or CV through join.pitt.edu, requisition number: 19004574
Master's degree or foreign equivalent degree in Biology, Biochemistry or related scientific field and three (3) years of experience with biochemical or biological laboratory research. Experience must include at least two years of experience with each of the following:
* Molecular, cellular, and immunohistological methods including cell culture, genotyping and maintaining knockout mice, single cell RT-PCR, ELISA, flow cytometry, protein quantification, western blotting and/or fluorescence microscopy;
* Identification of suitable strategies for visualization of targets of interest, verification of antibody specificity and establishing ELISAs for protein quantification;
* Demonstrated knowledge* of federal, state and local laws and regulations which may impact research undertaken in the laboratory.
References required. The following PA Act 153 clearances and background checks may be required prior to commencement of employment and as a condition of continued employment: PA State Police Criminal Record Check, FBI Criminal Record Check, PA Child Abuse History Clearance.
*Knowledge may be demonstrated through education, training and/or hands-on experience.
Assignment Category: Fulltime-Regular
Minimum Education Level Required: Master's
Minimum Experience Level Required: 3-5 years experience
Hiring Range: TBD based on qualifications
Background Check: For position finalists, employment with the University will require successful completion of a background check
Child Protection Clearances: Not Applicable
Required Documents: Resume
Optional Documents: Cover Letter
Internal Number: 19004574
About University of Pittsburgh
Founded in 1787, the same year the U.S. Constitution was signed, the University of Pittsburgh is one of the oldest institutions of higher education in the United States. One of three state-related research universities in Pennsylvania, Pitt is a member of the Association of American Universities (AAU), which comprises 62 preeminent doctorate-granting research institutions in North America.Pitt faculty members have expanded knowledge in the humanities and sciences, earning such prestigious honors as the National Medal of Science, the MacArthur Foundation’s “genius” grant, the Lasker-DeBakey Clinical Medical Research Award, and election to the National Academy of Sciences and the Institute of Medicine. Pitt scientists have defeated polio, unlocked the secrets of DNA, lead the world in organ transplantation, and pioneered TV and heavier-than-air flight, among numerous other accomplishments.Pitt students have earned Rhodes, Goldwater, Marshall, and Truman Scholarships, among other highly competitive national and international scholarships.Alumni have pioneered MRI and TV, won Nobel and Pulitzer Prizes, led corporations and universities, served in government and the military, conquered Hollywood and The New York Times bestsellers list, and won Super Bowls and NBA championships.