Reporting to Principal Investigator, the Laboratory Manager will be responsible for the management and operation of assigned laboratory. Included in that responsibility will be performing independent research projects, developing and implementing assays, protocols and operating procedures and the maintenance of genetically defined mouse colonies and bacteria colonies. Responsible for monitoring the adherence to JHU, departmental and animal use and care policies and procedures. Performs other related administrative and professional duties. The Laboratory Manager will provide supervision and technical assistance to research study staff on all aspects of laboratory operations, animal procedures and welfare, data collection, data management, and analysis with a high level of independence and minimal supervision. The Laboratory Manager will be responsible for Institutional Review Boards (IRB) matters, including communications with the IRB and coordinating the maintenance of study regulatory files and site visits with sponsors. The Laboratory Manager will direct preparation of reports and will work with PI for writing manuscripts and funding applications. Responsible for the efficient utilization of assigned resources and for planning future research and development, staffing, equipment and space. Develops capital and operating budget requirements designed to meet current and future needs of the laboratories.
Specific Duties & Responsibilities:
Perform independent research projects experiments involving an orthopaedic implant infection in mice and different models of Staphylococcus aureus, E. coli and P. aeruginosa infections, including a skin infection model, subcutaneous abscess model and a myositis model. This work involves performing animal survival surgery, performing noninvasive and longitudinal in vivo bioluminescence and fluorescence imaging, counting ex vivo bacterial colony forming units, performing high-resolution X-rays, micro-CT imaging, histology, microscopy, microbiological techniques, flow cytometry, ELISAs, protein arrays and quantitative real-time PCR arrays.
Perform independent research involving in vivo models of wound healing. These include models of Staphylococcus aureus and Pseudomonas aeruginosa infections in both wild type and diabetic mice as well as in wound healing under sterile conditions including detailed analysis of wound closure, granulation tissue formation and angiogenesis.
Perform independent experiments to study host-bacterial interactions and biofilm formation in various different in vitro culture systems. These systems will include the use of wild type and mutant bacterial strains to investigate the important bacterial factors that influence biofilm formation, bacterial virulence and growth. In addition, she/he will evaluate how these bacterial factors influence activation and survival of host cells in response to the bacteria.
Make significant and original contributions of the experiments described above. The candidate will be the primary person to plan experiments, present results, troubleshoot experimental difficulties and present the research findings in poster and oral presentations. The candidate will work closely with the principal investigator in all of these areas on a weekly basis. She/he is also expected to be a first-author on manuscripts produced from this work and she/he will make major contributions in writing these manuscripts.
Supervising, orienting and training other laboratory personnel, including post-doctoral fellows, medical and graduate students, Research Specialists, Research Technologists and Laboratory Technicians in Dr. Miller’s Laboratory. In this role I both teach laboratory techniques, assign laboratory maintenance duties and also promote their career development.
Troubleshooting and preventative maintenance of all laboratory equipment.
Preparing all documentation and assigned laboratory area for IRB, ACUC, and/or other regulatory reviews.
Managing both mouse and bacteria inventories.
Supervising and participating the ordering of supplies and acquisition of equipment and procurement of service contracts.
Organizing the lab meetings
Minimum Qualifications (mandatory):
Bachelor’s degree in biological sciences, chemistry, or physics and 5 years of related research laboratory experience required performing relevant techniques. Proven technical knowledge and laboratory experience required. Knowledge of good lab practices required. Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
JHU Equivalency Formula: 18 graduate degree credits may substitute for one year of experience. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
Master’s degree with related graduate research or candidates with a Ph.D. degree or that have completed a post-doctoral fellowship in a related field are preferred.
Special Knowledge, Skills, and Abilities:
Must have a working knowledge of one or more of the following disciplines achieved through undergraduate studies, and laboratory experience gained through class work: biology, biochemistry, microbiology and/or genetics. Two years of related research laboratory experience required performing relevant techniques.
This position requires a candidate that can perform independent research involved in a major research project involving the study of the protective immune responses, the pathogenesis of infection and the virulence factors associated with the human bacterial pathogen Staphylococcus aureus. This involves study of various different in vivo and in vitro infection models of Staphylococcus aureus infections, including skin infections, wound infections, systemic infections and infection of implanted medical/surgical devices and prostheses. The candidate will be responsible for performing various experimental procedures that include a working knowledge of microbiology, immunology and molecular biology techniques. The experimental procedures include: in vivo models of Staphylococcus aureus infections that utilize in vivo bioluminescence and fluorescence imaging, human organotypic cell cultures, various other human and mouse cell cultures, isolation of mouse and human cells from peripheral blood, isolation of mouse and human cells from the skin, immunohistochemistry, immunoassays (ELISA, myeloperoxidase assays), molecular biology techniques such as isolation of RNA, RT-PCR, real-time PCR, gel electrophoresis, microbiology techniques needed to grow and harvest Staphylococcus aureus, affinity column purification, FACS/Flow cytometry, intracellular staining, and protein analysis and quantification (e.g. western blots). In addition, this position will involve transfecting cells with various constructs to enable cells to overexpress or inhibit expression of various factors involved in the immune response. This position will also involve the development of various different antimicrobial and immune-based therapeutic strategies to help prevent or treat Staphylococcus aureus infections.
Classified Title: Laboratory Manager Working Title: Laboratory Manager Role/Level/Range: ACRP/04/MD Starting Salary Range: $51,975 - $71,497 Employee Group: Full Time Schedule:Mon – Fri, 8:30am – 5pm Exempt Status: Exempt Location: 1550 Orleans St Department Name: 10002833-SOM Der Research Division Personnel Area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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