Classified Title: Lab Technician Working Title: Lab Technician Role/Level/Range: ACRO40/E/02/CB Starting Salary Range: $12.76-$16.77 Employee group: Full-Time Schedule: M-F 8:30 am - 5:00 pm Exempt Status: Non-Exempt Location: 04-MD:School of Medicine Campus Department name: 10003524-SOM Neuro Neuromuscular Personnel area: School of Medicine
A technician is sought in the Department of Neurology. This laboratory studies the pathogenesis of a subtype of Charcot-Marie-Tooth disease (CMT), which is an inherited form of peripheral neuropathy and constitutes the most common inherited neurologic disorder worldwide. The laboratory focuses on CMT type 2C, which is caused by dominant mutations in the ion channel TRPV4 and causes weakness of the arms and legs, sensory loss, and respiratory difficulties. The laboratory studies fundamental functions of TRPV4 and how mutations disrupt these functions. The ultimate goal of these pursuits is to use cell culture and animal models for development of therapeutic strategies for this disease. The responsibilities of the individual in this position will be to assist in ongoing pursuits directed by the principal investigator and to independently pursue new projects related to the pathogenesis of TRPV4. In addition to performing laboratory experiments, responsibilities will include the isolation and culture of primary neurons, maintenance of immortalized cultured cells, generation of recombinant DNA expression vectors, assistance with management of transgenic mouse colonies, and animal drug treatment, behavioral testing, and surgeries. The ideal candidate will be highly motivated with exceptional personal initiative and communication skills.
Specific duties & responsibilities:
Laboratory experiments (transient transfection, Western blotting, co-immunoprecipitation, immunohistochemistry, culture of immortalized cells) (40%), isolation of primary mouse neurons (30%), mouse colony management, animal treatment, behavioral testing, and surgery (20%), and administrative duties (e.g., ordering reagents) (10%)
Minimum qualifications (mandatory):
High school diploma/GED with some science coursework required. Experience with molecular biology and biochemistry laboratory approaches is helpful, but not required.
The successful candidate(s) for this position will be subject to a pre-employment background check.
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