Department name: 10002833-SOM Der Research Division Schedule: M-F, 8:30 am - 5:00 pm Location: 04-MD:School of Medicine Campus Personnel area: School of Medicine
The Research Specialist will work in a laboratory with an emphasis on Dermatology using both mouse and human tissues. Develops assays and protocols for PI approval and design experimental approaches for research projects. Performs a wide range of sophisticated assays. Make significant/important contributions to various aspects of research efforts. Work includes troubleshooting, independent decisions and training/guidance of other lab personnel. In general, the candidate will be responsible for helping to manage day-to-day lab operations and will participate in research projects by other laboratory members, including, post-doctoral fellows, laboratory technicians and undergraduate, graduate and medical students working in the laboratory.
SPECIFIC DUTIES & RESPONSIBILITIES
Essential Job Functions:
Makes scientific contribution to protocol development, efficiency, and productivity. Collaborates in development of new techniques and instruments.
Responsible for assigned daily research projects. Utilizes specialized knowledge to interpret results, e.g. reading controls, evaluate results.
Acts as the Core supervisor for data generated for the lab as assigned. Develops, organizes, and provides resources related to research including tissues, mouse models, and material for primary cell culture, available on a national and international scale. Trains laboratory staff, graduate students, and outside investigators in skills related to experiments and related analyses.
Uses software applications to assemble, manipulate, analyze and present data.
Orders equipment and supplies, interfaces with vendors.
Uses scientific knowledge and lab experience to make judgments and decisions. Works independently.
Teaches/trains students, and new technicians in the lab. Guides, directs and facilitates problem solving for entry-level lab members on day-to-day problems with more challenging research assays.
Applies published research methods to current experimental work by investigating published protocols and discussing them with PI.
Scientifically contributes to writing of manuscripts and grants. Processes analyzed data into figures for scientific publications.
Helps to perform various experimental procedures that include a working knowledge of microbiology, immunology and molecular biology techniques. The experimental procedures include: in vivo models of Staphylococcus aureus infections that utilize in vivo bioluminescence and fluorescence imaging, human organotypic cell cultures, various other human and mouse cell cultures, isolation of mouse and human cells from peripheral blood, isolation of mouse and human cells from the skin, immunohistochemistry, immunoassays (ELISA, myeloperoxidase assays), molecular biology techniques such as isolation of RNA, RT-PCR, real-time PCR, gel electrophoresis, microbiology techniques needed to grow and harvest Staphylococcus aureus, affinity column purification, FACS/Flow cytometry, intracellular staining, and protein analysis and quantification (e.g. western blots).
Transfects cells with various constructs to enable cells to overexpress or inhibit expression of various factors involved in the immune response.
Develops various different antimicrobial and immune-based therapeutic strategies to help prevent or treat Staphylococcus aureus infections.
Uses sterile techniques to avoid contaminating lab experiments.
Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens. Complies with biohazard/radiation safety standards through proper handling of potentially hazardous chemical and biological agents and/or radiation sources. Completes annual university biohazard/universal precaution/radiation safety training, as appropriate, as well as other required online and in-person trainings and compliance requirements.
Monitors equipment performance and maintenance schedules. May perform routine repair or replacement of standard parts.
Scope of Responsibility
Knows the informal policies, procedures and practices necessary to conduct the normal function of a specific section, unit, or work area. Is aware of the role of the position and its potential impact on the working unit, and within the Department. Functions as a member of a team, working to promote an atmosphere of respect, collegiality, integrity and discovery.
While this individual does not directly supervise other staff, he/she is sought for advice and guidance from a variety of junior laboratory staff, students, and fellows in projects and analysis.
On a regular and continuous basis, follows experimental protocols, complies with biohazard/radiation safety standards. Engages in regular and continuous decision making having an impact on people, costs, and/or quality of service.
Carries out duties and responsibilities with limited supervision. Makes decisions and establishes work priorities on essentially procedure-oriented operations. Exchanges non-routine information using tact and persuasion as appropriate requiring good oral and written communications skills. Practices service recovery and demonstrates service excellence in all aspects of the job.
BS/BA or equivalent required, in biological sciences, chemistry, or physics and one year of related research laboratory experience performing relevant research laboratory experince required. Must have a working knowledge of one or more of the following disciplines achieved through undergraduate studies, and laboratory experience gained through class work: biology, biochemistry, physics, microbiology and/or genetics. Knowledge of good lab practices required.
This position requires a candidate that can perform independent research involving the of role bacterial-host interactions in inflammatory skin diseases such as atopic dermatitis. This involves study of various different in vivo and in vitro infection models of Staphylococcus aureus infections and skin inflammation. The candidate will be responsible for performing various experimental procedures that include a working knowledge of microbiology, immunology and molecular biology techniques. The experimental procedures include: in vivo models of Staphylococcus aureus infections that utilize in vivo bioluminescence and fluorescence imaging, human organotypic cell cultures, various other human and mouse cell cultures, isolation of mouse and human cells from peripheral blood, isolation of mouse and human cells from the skin, immunohistochemistry, immunoassays (ELISA, myeloperoxidase assays), molecular biology techniques such as isolation of RNA, RT-PCR, real-time PCR, gel electrophoresis, microbiology techniques needed to isolate, grow and harvest Staphylococcus aureus and other colonizing bacteria, affinity column purification, FACS/Flow cytometry, intracellular staining, and protein analysis and quantification (e.g. western blots). This position will also involve the development of various different antimicrobial and immune-based therapeutic strategies to help prevent or treat Staphylococcus aureus infections or skin inflammation. Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
JHU Equivalency Formula: 18 graduate degree credits may substitute for one year of experience.
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at firstname.lastname@example.org. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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