Classified Title: Research Specialist II Working Title: Role/Level/Range: ACRP/03/MB Starting Salary Range: $38,536-$52,989 (commensurate with experience) Employee group: Full-Time Schedule: Working 37.5 hours per week, Monday-Friday 8:30-5pm Exempt Status: Exempt Location: 04-MD:School of Medicine Campus Department name: 10003523-SOM Neuro Neurological Oncology Personnel area: School of Medicine
The position is responsible for setting up a new laboratory studying innovative treatments for brain tumors and NF1. The ideal applicant will be an experienced Scientist with a strong background in AAV viral vector production and associated analytics, as well as, knowledge in T-cell biology, Immunology, and Oncology.
Specific Duties & Responsibilities:
This position is responsible for production of recombinant adeno-associated virus (rAAV) vectors for gene replacement therapy in NF1 via maintenance of mammalian cell lines, transfection, infection, and downstream purification of rAAV by chromatography and ultracentrifugation. The individual will be involved in scalable downstream purification technologies for rAAV using FPLC systems, tangential flow filtration, and centrifugation. In addition, he/she will genetically modify isolated T cells with CAR constructs and be responsible for their propagation and maintenance.
Supervises, orients, and trains staff of the laboratory. Insures funder and JHU requirements are met.
Participates in the specification, design, implementation, and modification of projects related to the lab. Oversees and participates in study record management, and data collection. Assists with preparation of technical reports, manuscripts, abstracts, presentations, and funding applications. Performs complex and routine experiments following both established and new protocols.
Responsible for compliance and audit preparation of assigned laboratory area for IRB, ACUC,CLIA, CAP, The Joint Commission, and/or other regulatory reviews. Coordinates and maintains all regulatory binders, logs and audits.
Ordering of supplies and acquisition of equipment and procurement of service contracts.
Organize and lead assigned meetings. Attendance in meetings (including national), as required.
Maintain facilities, repair and maintenance of laboratory and office equipment.
Design, develop and test novel plasmid constructs to enhance AAV production, tropism and transgene expression.
Define, develop and employ innovative techniques to characterize AAV vectors in vitro and in vivo.
Develop medium/large scale in-house AAV production processes
Maintaining cell growth, transfection, of mammalian cells for rAAV
Execution of downstream purification technologies related to rAAV purification using chromatography (affinity and ion-exchange) and filtration processes including microfiltration, tangential flow filtration.
Perform vector characterization assays (qPCR, SDS-PAGE, Western blotting, etc.) on in-process and final samples on as needed basis
Preparing protocols and reports in notebooks and electronic documents
Minimum Qualifications (Mandatory):
Bachelor’s degree in biology, chemistry, or other related field, and a minimum of 2 years of experience in biology, chemistry, or related field required. Additional education may substitute for some experience, to the extent permitted by the JHU equivalency formula.
Graduate degree in Virology or Molecular Biology with a focus on Virology
Special knowledge, skills, and abilities:
Extensive experience designing and producing AAV vectors
Proficiency with a broad spectrum of viral vector specific analytical techniques
Experience with medium/large scale AAV production
Experience and working knowledge of concepts including but not limited to genetic engineering, molecular biology techniques, cell biology, AAV gene therapy, protein analysis, ELISA, etc.
Experience in immune cell isolation.
Experience in functional assays measuring cytokine release.
Demonstrated problem-solving skills.
Organized, detail-oriented, and a self-starter who continuously seeks to improve existing laboratory methods and processes.
Demonstrated ability to operate and maintain laboratory equipment in a specialty lab. Computer competency is required.
Excellent communication skills with the proven ability to build open and collaborative relationships and work effectively as a member of a multidisciplinary team.
Experience coordinating, conducting and managing laboratory studies. Experience with IRB and ACUC regulatory matters
The successful candidate(s) for this position will be subject to a pre-employment background check.
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During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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