This is a limited appointment may be extended or converted to career status based on the needs of the department.
The Department of Neurosciences has a large research group studying neuro-degenerative diseases. Stimulated by neuropathological observations and genetic discoveries, studies in cell and animal models of neuro-degenerative disorders have begun to clarify pathogenetic mechanisms of disease. We examine the intersection of these research themes and identify several potential mechanisms for linking failed neurotrophic factor signaling to neurodegeneration. We will be expanding our research group and are looking for a Staff Research Associate to support our efforts.
Incumbent will be independently responsible for the dissection of fresh or fixed human brains including tissue preparation and processing, fulfilling tissue requests, logging and recording specimen data, as well as overseeing Alzheimer's Disease and Neurodegeneration tissue/brain bank. Incumbent will carry out experiments aimed at understanding the molecular mechanisms involved in neurodegeneration in neurological disorders such as Alzheimer's Disease, Parkinson's Disease and HIV including tissue culture analysis followed by real time PCR, Western blots, immunoprecipitation and cell assays. Additional duties include maintain lab stocks of chemicals and supplies, accurate and detailed lab notebook and other records, and attending weekly lab meetings.
Theoretical knowledge in cell biology, chemistry and molecular genetics as typically acquired by a Bachelor's Degree; or an equivalent combination of education and experience.
Ability to comprehend and discuss research materials. Proven experience performing accurate record keeping and organize and calculate data. Skill to plan and organize work assignments in a efficient and productive manner. Ability to maintain meticulous records regarding all aspects of work performed in the lab.
Demonstrated experience in molecular biology including: RNA/DNA isolation, Real-time PCR, bacterial transformation, Western blot, and tissue culture.
Proven experience producing high quality vibratome sections for confocal microscopy and high quality thin and semi-thin sections for electron microscopy.
Demonstrated experience processing human and murine brain tissue for immunocytochemical assays and western blots, and electron microscope.
Experience with image analysis. Basic imaging skills and ability to perform Electron Microscopy (EM), immunogold chemistry, prepare staining solutions, and perform Electron microcopy analysis. Ability to learn new and increase skills in imaging techniques.
Knowledge of neurological disorders such as Alzheimer's Disease, Parkinson's Disease and HIV.
Experience with and knowledge of the operation and maintenance of various laboratory equipment such as confocal, fluorescent, and electron microscopes, centrifuges, plate readers, microtomes, and vibratomes.
Demonstrated experience preparing buffers and solutions.
Demonstrated experience in genotyping and Fluorescence-Activated cell sorting.
Strong computer skills and experience with the operation of all basic software application such as Word and Excel, ability to work with a variety of statistical and analysis programs, and learn specialized imaging and analysis software.
Excellent communication skills (verbally and written) to interact professionally and effectively in a basic research work environment, and accurately document research procedures that facilitate professional and effective interaction with diverse population.
Strong interpersonal skills including tact, diplomacy and flexibility. Ability to work independently and/or in a team framework in conjunction with principles of community and in a lab environment that interacts with a diverse group of research and technical personnel and other laboratories.
Knowledge of basic lab safety requirements, procedures, and ability to follow EH&S regulations and methods for handling, inventory, storing, clean-up, and waste disposal for biohazardous materials. Experience and knowledge to read and use Material Safety Data Sheets.
Ability to maintain inventories and order supplies for lab. Ability to maintain safe and orderly laboratory. Excellent organizational skills, including ability to multitask. Ability to prioritize workload and to shift efforts to meet changing and demanding project and research timelines.
Knowledge of UCSD Environmental Health and Safety requirements including radiation and biosafety procedures.
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