Classified Title: Research Technologist Working Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: [[$ 28,558.32 - 39,249.60]] Employee group: Full Time Schedule: M-F/8 Exempt Status: Non-Exempt Location: [[855 N. Wolfe Street]] Department name: 60003628-Neurodegenerative and Injury Program Personnel area: School of Medicine
The job will employ central nervous system post mortem tissue derived samples from a range of human neurological diseases including amyotrophic lateral sclerosis and animal models (mice) that develop these diseases.
The technician will become proficient in the novel and modern methods of immunofluorescence, immunohistochemistry and microscopy, and cell type specific isolation (neurons, astroglia) from brain and spinal cord samples.
We are looking for a proficient research laboratory specialist with a sophisticated understanding of techniques and a solid foundation in the underlying science, gained from professional experience. The individual will work in close collaboration with a research associate who will manage the research projects and will guide the research technologist on a regular basis.
The research technologist will participate in planning experiments and evaluating/interpreting test results utilizing detailed knowledge of research laboratory procedures and practices. The individual should be capable of independent judgment in the selection, development, performance and evaluation of research methods; operates appropriate equipment.
The primary duties and responsibilities of the job:
Follows established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments which include: animal nervous system tissue harvesting (Brain and spinal cord), RNA and protein isolation, immunohistochemistry/immunofluorescence and imaging of human and mouse CNS derived samples. Conventional RNA and protein analysis including real time PCR and Western Blot.
Uses various software applications, such as Excel spreadsheets, PowerPoint, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports.
Participate in the organization and operation of the laboratory. Performs general lab maintenance duties, may need to order and manage supplies and reagents of the lab. Will need to maintain excellent written records and electronic databases. Prepares solutions, reagents, and stains following standard laboratory formulas and procedures.
Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens. Completes annual university biohazard/universal precaution/radiation safety training, as appropriate. Monitors biohazard/radiation safety standards and proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace. Monitors inventory levels and proper storage of materials and supplies in accordance with established policies and procedures. Keeps current knowledge of vendors and suppliers of materials specific to the laboratory requirements.
Bachelor's degree, or equivalent, in biology, chemistry or related field. Six months related experience.
Experience with histology, light microscopy, immunohistochemistry, animal (mouse) tissue dissection.
We welcome anybody who is talented, creative and highly motivated. A background in immunohistochemistry would be preferred, but is not required.
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at email@example.com. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.